Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kristen J Lindblad
Date created: 2008-07-27 11:00:00
Date modified: 2015-05-08 01:08:42
Dual-Repressed Promoter for p22 Mnt and TetR with RBS (med strength)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K101009_sequence (Version 1) |
Description
This promoter will be repressed in the presence of the Mnt repressor from the P22 phage, the Tet repressor from E. coli, or both. A medium strength RBS has been incorporated for convenient cloning to a desired gene.Notes
The dually-repressed promoter contains a TetR operator site derived from the pTet promoter of E. coli upstream of the -35 polymerase binding site, and a P22 Mnt operator site between the -35 and -10 polymerase binding sites. The -35 and -10 binding site were derived from the pTet promoter. For information concerning the RBS design, please see part BBa_B0032.Source
This part combines BBa_K101000, the dually-repressed promoter, with BBa_B0032, a medium strength RBS with good characterization and experience by other registry users.| Access | Instance | Definition |
| public public | BBa_K101000 BBa_B0032 | BBa_K101000 BBa_B0032 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K101000 BBa_B0032 | 1,61 70,82 | BBa_K101000 BBa_B0032 |