Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bennett Swiniarski
Date created: 2008-07-27 11:00:00
Date modified: 2015-05-08 01:08:42
Dual-Repressed Promoter for p22 cII and TetR with RBS attached
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K101010_sequence (Version 1) |
Description
This composite part consists of a dually-repressed promoter with a medium strength RBS attached. The promoter is repressed by either p22 cII or TetR proteins. The medium strength RBS was attached to make this promoter easier to use with any protein that may need to be attached to it. When the RBS is already on the promoter, a simpler ligation can occur that attaches the protein to this promoter, instead of having to attach a small RBS before this ligation can occur.Notes
There were no real design considerations needed for this part. All that was done was to use PCR to add the small RBS fragment to the dual promoter construct.Source
This part was constructed from BioBrick parts BBa_K101002 (Dual Promoter) and BBa_B0032 (medium strength RBS).| Access | Instance | Definition |
| public public | BBa_K101002 BBa_B0032 | BBa_K101002 BBa_B0032 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K101002 BBa_B0032 | 1,66 75,87 | BBa_K101002 BBa_B0032 |