Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kristen J Lindblad
Date created: 2008-07-27 11:00:00
Date modified: 2015-05-08 01:08:42
Dual-Repressed Promoter for LacI and Lambda cI + RBS (med strength)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K101011_sequence (Version 1) |
Description
This promoter will be repressed in the presence of the LacI protein from E. coli, the cI protein from the Lambda phage, or both. A medium strength RBS has been incorporated for convenient cloning to a desired gene.Notes
This promoter contains two lambda cI operator sites; one upstream of the -35 polymerase binding site, and another between the -35 and -10 binding sites. It also includes a LacI operator site downstream of the -10 polymerase binding site. -35 and -10 sites were derived from the cI repressed promoter from lambda phage. A CAP binding site is also included for effective repression. For information concerning the RBS design, please see part BBa_B0032.Source
This part combines BBa_K101001, the dually-repressed promoter, with BBa_B0032, a medium strength RBS with good characterization and experience by other registry users.| Access | Instance | Definition |
| public public | BBa_K101001 BBa_B0032 | BBa_K101001 BBa_B0032 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K101001 BBa_B0032 | 1,116 125,137 | BBa_K101001 BBa_B0032 |