BBa_K101014

BBa_K101014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K101014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yiannis Kaznessis

Date created: 2008-10-19 11:00:00

Date modified: 2015-05-08 01:08:42

AraC promoter, RBS, GFP and terminator

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K101014_sequence (Version 1)

Description

A previously not characterized promoter was characterized using the standard promoter characterization technique. The promoter R0080 was cloned with RBS-GFP-terminator (E0240). The GFP expression was measured and found to be weak compared to promoter J61002.

Notes

R0080 digested with EcoRI and SPI. E0240 digested with xBAI and PSTI and ligated to EcoRI and PSTI cut pSB3K3

Source

BioBricks

Components

Access	Instance	Definition
public	BBa_R0080	AraC
public	BBa_B0032	BBa_B0032
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0080	1,149	AraC
BBa_B0032	158,170	BBa_B0032
BBa_E0040	177,896	GFP
BBa_B0010	905,984	BBa_B0010
BBa_B0012	993,1033	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K101014/1

Member of these Collections

• iGEM Parts Registry