Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Karl Petersen
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:08:42
MioC Promoter (DNAa-Repressed Promoter)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K101017_sequence (Version 1) |
Description
The MioC promoter is a cell-cycle dependent promoter that is repressed before initiation of replication and depressed shortly after. The regulation of the MioC gene is overseen by several DNAa proteins, which bind along the promoter. This particular MioC promoter was isolated from K-12 (JM109).This promoter can be used in series with a reporter to signal only during a given part of the cell cycle.
Notes
Site directed mutagenesis was used to create a Spe1 restriction site.Source
The source of this part is from the genomic dna of E.coli (JM109).| Sequence Annotation | Location | Component / Role(s) |
| DNAa Binding Box DNAa Binding Box DNAa Binding Box DNAa Binding Box MioC Promoter | 295,303 283,292 273,279 159,167 131,319 | feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/promoter promoter |