BBa_K1012002

BBa_K1012002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1012002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Allan
Date created: 2013-08-28 11:00:00
Date modified: 2015-05-08 01:08:42

The TorA twin-arginine signal sequence



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1012002_sequence (Version 1)

    Description

    Protein phosphatase 1 (PP1,human protein) with N terminal TorA signal sequence and C terminal hemagglutinin (HA) tag.

    TorA signal sequence is taken from E. coli TMAO reductase. This will target the protein for Tat transport.

    HA tag is attached for identification of PP1 with Western blots.

    Notes

    PP1 contained PstI site, this was removed by quick change.

    TorA signal sequence was added by standard cloning proceudres.

    Source

    Published paper, reference needed

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