BBa_K1012004

BBa_K1012004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1012004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Allan

Date created: 2013-08-28 11:00:00

Date modified: 2015-05-08 01:08:42

The maltose binding protein (MalE) signal sequence

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1012004_sequence (Version 1)

Description

Protein phosphatase 1 (PP1, human protein) with N terminal MalE signal sequence, and C terminal hemagglutinin tag.

MalE signal sequence is taken from E. coli maltose binding protein. This signal will target the protein for Sec transport.

HA tag can be used for identification of PP1 by Western blot.

Notes

PP1 contained a PstI site which was removed by quick change.

MalE signal sequence was added by standard cloning methods.

Source

Published paper, reference needed

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1012004/1

Member of these Collections

• iGEM Parts Registry