BBa_K1012005

BBa_K1012005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1012005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Allan

Date created: 2013-08-28 11:00:00

Date modified: 2015-05-08 01:08:42

GFP under control of an OmpR-responsive promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1012005_sequence (Version 1)

Description

Reperter gene for the EnvZ receptor. Contains binding site for OmpR, a strong ribosome binding site and green fluorescent protein (GFP).

EnvZ phosphorylation of OmpR results in binding of OmpR to this composite part leading to expression of GFP.

Notes

This composite part was assembled using standard cloning methods.

Source

Registry, DNA distribution kit

Components

Access	Instance	Definition
public	BBa_R0083	OmpR
public	BBa_B0034	BBa_B0034
public	BBa_E0040	GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0083	1,78	OmpR
BBa_B0034	87,98	BBa_B0034
BBa_E0040	105,824	GFP

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1012005/1

Member of these Collections

• iGEM Parts Registry