| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1019000_sequence (Version 1)
|
Description
"FimB, along with FimE, is a recombinase in Escherichia coli which catalyzes the site-specific recombination required for inversion of a 314-bp invertible DNA element, the fim switch (fimS), to control transcription of the type I fimbrial structural genes--a process known as phase-variation switching. fimS contains the promoter for expression of the fimbrial structural genes. The promoter is positioned to allow expression of fimAICDFGH when fimS is in the ON orientation, but not when it is in the OFF orientation. FimB is responsible for both OFF-to-ON and ON-to-OFF switching at equal rates. FimE is responsible for primarily ON-to-OFF switching." (http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10309)
Notes
The gene was isolated using PCR and cloned into our expression vector pEBS where it is under inducible control via the lac promoter.
Source
E. coli MG1655 Genome
