Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Boris Dyakov
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:43
pEBS Expression Plasmid
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_K1019008_sequence (Version 1) |
Description
pEBS (E.coli Biofilm System) is an expression plasmid with an inducible lac promoter. It contains the biobrick prefix and suffix, an RBS, and a unique BseRI cut site which linearizes the vector for use with the Clontech In-Fusion restriction free cloning system. This system allows any gene with the appropriate homology region added to its start and end to be easily swapped into the vector for testing.Notes
The plasmid was created by excising the maltose binding domain of the original pMAL plasmid and then using In-Fusion cloning to insert a consensus region containing the features needed.Source
Derived from the pMAL plasmid.| Sequence Annotation | Location | Component / Role(s) |
| ColE1 origin F1 origin M13 origin Amp Resistance LacZ alpha LacO RBS M13F Start LacI rrnB T1 terminator rrnB T2 terminator 2x ochre STOP Prefix suffix M13R | 3716,4398 3358,3664 3226,3681 2464,3123 1672,1740 1438,1460 1549,1560 1599,1616 1561,1563 81,1163 1941,2020 2127,2155 1564,1569 1529,1548 1571,1590 1450,1469 | sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature CDS feature/cds CDS feature/cds operator feature/operator feature/rbs ribosome_entry_site primer_binding_site feature/primer_binding start_codon feature/start feature/cds CDS feature/misc sequence_feature feature/misc sequence_feature feature/stop stop_codon feature/misc sequence_feature sequence_feature feature/misc feature/primer_binding primer_binding_site |