Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yue Liu
Date created: 2013-08-17 11:00:00
Date modified: 2015-05-08 01:08:43
AlkR -PSB1C3-Low constitutive
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1020003_sequence (Version 1) |
Description
AlkR is a transcriptional regulator belonging to AraC/Xyls family, which could detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. It is said that AlkR is the only bioreporter that is able to detect alkane with carbon chain length greater than C18.The mechanism is that alkanes are presumably recognized by AlkR regulatory protein and their interaction triggered a conformation change of AlkR dimers which isomerizes the promoter-RNAP complex and lead to the activation of alkM gene.
AlkR regulatory protein includes C-terminal DNA-binding domain(CTD) for promoter binding and N-terminal domain(NTD) for inducer recognition.
Here we use a constitutive promoter for AlkR gene and in case of interference, we choose a long and strong terminator.
Notes
We use a constitutive promoter for AlkR gene and we choose a long and strong terminator to prevent interference.Source
Acinetobacter baylyi ADP1| Access | Instance | Definition |
| public public public public | BBa_J23103 BBa_B0034 BBa_K1020002 BBa_B0015 | BBa_J23103 BBa_B0034 BBa_K1020002 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23103 BBa_B0034 BBa_K1020002 BBa_B0015 | 1,35 44,55 62,988 997,1125 | BBa_J23103 BBa_B0034 BBa_K1020002 BBa_B0015 |