Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yue Liu
Date created: 2013-08-17 11:00:00
Date modified: 2015-05-08 01:08:43
Biosensor for alkane detection
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1020004_sequence (Version 1) |
Description
AlkR is a transcriptional regulator belonging to AraC/Xyls family, which could detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. It is said that AlkR is the only bioreporter that is able to detect alkane with carbon chain length greater than C18.The mechanism is that Alkane were presumably recognized by AlkR regulatory protein and their interaction triggered a conformation change of AlkR dimers which isomerizes the promoter-RNAP complex and lead to the activation of alkM gene.
AlkR regulatory protein includes C-terminal DNA-binding domain(CTD) for promoter binding and N-terminal domain(NTD) for inducer recognition.
Notes
We choise a constitutive promoter for AlkR gene and in case of interference, we use a long and strong terminator.Source
Acinetobacter baylyi ADP1| Access | Instance | Definition |
| public public public public | BBa_K1020001 BBa_J23100 BBa_B0034 BBa_K1020002 | BBa_K1020001 BBa_J23100 BBa_B0034 BBa_K1020002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1020001 BBa_J23100 BBa_B0034 BBa_K1020002 | 1,1013 1022,1056 1065,1076 1083,2009 | BBa_K1020001 BBa_J23100 BBa_B0034 BBa_K1020002 |