Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maithili Krishnan
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:08:45
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II : RBS TetR :TT
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1022122_sequence (Version 1) |
Description
This biobrick is one of the final constructs in Project Peptidor by Team TU Delft iGEM 2013 to check the sensing of AIP by E.coli and then production of the peptides followed by TetR production to activated the next set of the constructs.Notes
The Biobrick Bba_K1022106 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119Source
Clonned| Sequence Annotation | Location | Component / Role(s) |
| promoter BBa_K206000 AraI1 AraI2 BBa_I746103 BBa_I746100 B0034-sequence RBS agrC coding sequence BamHI site B0034-sequence RBS agrA coding sequence BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_I746104 BBa_K1022119 pT7 His 6 - SUMO Magainin II 34 Tet R B0010 B0012 | 1,131 1,130 40,57 61,78 139,2299 139,2058 139,150 157,1404 1405,1410 1411,1422 1429,2058 2067,2146 2078,2121 2155,2195 2162,2181 2182,2195 2188,2188 2204,2299 1,2299 2300,2313 2314,2634 2638,2707 2712,2715 2729,3344 3419,3498 3507,3547 | feature/promoter promoter engineered_region feature/BioBrick operator feature/operator operator feature/operator engineered_region feature/BioBrick feature/BioBrick engineered_region feature/rbs ribosome_entry_site CDS feature/cds feature/misc sequence_feature ribosome_entry_site feature/rbs feature/cds CDS engineered_region feature/BioBrick stem_loop feature/stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon feature/BioBrick engineered_region feature/BioBrick engineered_region feature/promoter promoter tag feature/tag sequence_feature feature/dna ribosome_entry_site feature/rbs CDS feature/cds stop_codon feature/stop feature/stop stop_codon |