BBa_K1023017

BBa_K1023017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1023017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michiel Karrenbelt

Date created: 2013-12-19 12:00:00

Date modified: 2015-05-08 01:08:45

eForRed Chromoprotein

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1023017_sequence (Version 1)

Description

We synthesized and via Gibson assembly put together a chromoprotein encoding gene (eforRed) which was codon-optimized for Aspergillus niger.
A mitochondrial retention signal was added to this codon-optimized chromoprotein encoding gene for targeted organelle localization. There is a Nsi restriction site in the middle that allows one to cleave of the signal peptide if required. 5'-signal seq - Nsi1 - eforRed-3'

Notes

codon bias

Source

The XL1 Blue MRF??? E. coli strains which contain plasmids with chromoprotein encoding genes aeBlue, eforRed and amilGFP respectively and DH5α E. coli strain containing mRFP were obtained from Braunschweig UR and Uppsala UR.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Nsi1	73,79	CDS, feature/cds

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1023017/1

Member of these Collections

• iGEM Parts Registry