BBa_K1026003

BBa_K1026003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1026003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hongyi WU

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:08:46

gRNA targeting mRFP

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1026003_sequence (Version 1)

Description

A gRNA that targets mRFP reporter.
This gRNA sequence comes from the following literature:
QI, LEI S., LARSON, MATTHEW H., GILBERT, LUKE A., DOUDNA, JENNIFER A., WEISSMAN, JONATHAN S., ARKIN, ADAM P. & LIM, WENDELL A. 2013. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell, 152, 1173-1183.

Notes

Criteria for designing a gRNA in CRISPRi are illustrated in the above literature.

Source

The gRNA sequence is de novo synthesized.

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1026003/1

Member of these Collections

• iGEM Parts Registry