Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hongyi WU
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:46
Constitutively Expressed Red Light Sensor Operon
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1026005_sequence (Version 1) |
Description
This Constitutively Expressed Red Light Sensor Operon is an assembly of the constitutive promoter BBa_J23100, a strong RBS BBa_B0034, red light sensor Cph8 and a reliable terminator BBa_B0015. The blue light sensor utilized here consists of the Cph8-OmpR system (Levskaya et al., 2005, Tabor et al., 2011).Reference
LEVSKAYA, A., CHEVALIER, A. A., TABOR, J. J., SIMPSON, Z. B., LAVERY, L. A., LEVY, M., DAVIDSON, E. A., SCOURAS, A., ELLINGTON, A. D., MARCOTTE, E. M. & VOIGT, C. A. 2005. Synthetic biology: engineering Escherichia coli to see light. Nature, 438, 441-2.
TABOR, J. J., LEVSKAYA, A. & VOIGT, C. A. 2011. Multichromatic control of gene expression in escherichia coli. Journal of Molecular Biology, 405, 315-324.
Notes
The expression level of Red Light Sensor shall be delicately controlled, so as to acquire continuous adjustment (regulation) of target genes.Source
The red light sensor originates from cyanobacteria.| Access | Instance | Definition |
| public public public public | BBa_J23100 BBa_B0034 BBa_K592000 BBa_B0015 | BBa_J23100 BBa_B0034 cph8 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K592000 BBa_B0015 | 1,35 44,55 62,2299 2308,2436 | BBa_J23100 BBa_B0034 cph8 BBa_B0015 |