Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hongyi WU
Date created: 2013-09-26 11:00:00
Date modified: 2015-05-08 01:08:46
A rationally designed promoter negative regulated by CcaR
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1026009_sequence (Version 1) |
Description
By fusing G-box, a region that CcaR binds, with the constitutive promoter, BBa_J23107, we devised a promoter that is constitutive without active CcaR (the transcription factor in green light sensor system, CcaS-CcaR-PcpcG2), but be inhibited when CcaR is activated by green light.Notes
We discovered a segment around the -35 region of BBa_J23107 that is almost identical to G-box, the binding region of CcaR on PcpcG2.So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter.
Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization.