BBa_K1026011

BBa_K1026011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1026011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hongyi WU

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:08:46

Constitutively Expressed Blue Light Sensor Operon

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1026011_sequence (Version 1)

Description

This Constitutively Expressed Blue Light Sensor Operon is an assembly of the constitutive promoter BBa_J23100, a strong RBS BBa_B0034, coding sequence of dCas9 protein and a reliable terminator BBa_B0015.
The blue light sensor utilized here consists of the YF1-fixJ system (Moglich et al., 2009).

Reference
MOGLICH, A., AYERS, R. A. & MOFFAT, K. 2009. Design and signaling mechanism of light-regulated histidine kinases. J Mol Biol, 385, 1433-44.

Notes

The expression level of Blue Light Sensor shall be delicately controlled, so as to acquire continuous adjustment (regulation) of target genes.

Source

The sensor comes from cyanobacteria.

Components

Access	Instance	Definition
public	BBa_J23100	BBa_J23100
public	BBa_B0034	BBa_B0034
public	BBa_K592004	YF1
public	BBa_B0034	BBa_B0034
public	BBa_K592005	FixJ
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23100	1,35	BBa_J23100
BBa_B0034	44,55	BBa_B0034
BBa_K592004	62,1192	YF1
BBa_B0034	1201,1212	BBa_B0034
BBa_K592005	1219,1839	FixJ
BBa_B0015	1848,1976	BBa_B0015

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1026011/1

Member of these Collections

• iGEM Parts Registry