Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hongyi WU
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:46
Constitutively Expressed Blue Light Sensor Operon
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1026011_sequence (Version 1) |
Description
This Constitutively Expressed Blue Light Sensor Operon is an assembly of the constitutive promoter BBa_J23100, a strong RBS BBa_B0034, coding sequence of dCas9 protein and a reliable terminator BBa_B0015.The blue light sensor utilized here consists of the YF1-fixJ system (Moglich et al., 2009).
Reference
MOGLICH, A., AYERS, R. A. & MOFFAT, K. 2009. Design and signaling mechanism of light-regulated histidine kinases. J Mol Biol, 385, 1433-44.
Notes
The expression level of Blue Light Sensor shall be delicately controlled, so as to acquire continuous adjustment (regulation) of target genes.Source
The sensor comes from cyanobacteria.| Access | Instance | Definition |
| public public public public public public | BBa_J23100 BBa_B0034 BBa_K592004 BBa_B0034 BBa_K592005 BBa_B0015 | BBa_J23100 BBa_B0034 YF1 BBa_B0034 FixJ BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K592004 BBa_B0034 BBa_K592005 BBa_B0015 | 1,35 44,55 62,1192 1201,1212 1219,1839 1848,1976 | BBa_J23100 BBa_B0034 YF1 BBa_B0034 FixJ BBa_B0015 |