Si4

BBa_K1028000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1028000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oleg Karpov, Joseph Beton
Date created: 2013-08-29 11:00:00
Date modified: 2015-05-08 01:08:46

Silica binding peptide



Types
DnaRegion

Roles
tag

Tag

Sequences BBa_K1028000_sequence (Version 1)

Description

This is a short peptide of 15 amino acids designed to bind with high affinity to silica and silica-like particles, designed as a tag to be attached to the C-terminus of polypeptides. This part was originally designed to be conjugated to the C-terminal of Ice Nucleation Protein allowing extracellular expression of the binding region.

Notes

The sequence was codon optomised for E.Coli expression.

Source

This sequence is taken from the "Site-Specific Labeling of Surface Proteins on Living Cells Using Genetically Encoded Peptides that Bind Fluorescent Nanoparticle Probes" paper published by Rocco et al.

Sequence Annotation Location Component / Role(s)
stop codon
stop codon
Coding region
46,46
49,49
1,45
feature/stop stop_codon
feature/stop stop_codon
engineered_region feature/BioBrick
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1028000/1