| Types | DnaRegion
|
| Roles | Terminator
terminator
|
| Sequences | BBa_K102900_sequence (Version 1)
|
Description
TA0 is a null terminator with minimal secondary structure (energy = -7.1 kCal/mole). It is used to represent a TRUE (always ON) gate and should give the maximum output possible.
Notes
Gate is flanked by BamHI and NcoI to facilitate simple replacement in output test harness. Because gates are fairly long duplex DNA strands, a single strand can be ordered and the dsDNA created by PCR using Term+ (5'cgatcagaattcgcggcc) and Term- (5'GGACATCTGCAGCGGCCG) primers.
Source
Synthetic
