BBa_K102901

BBa_K102901 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K102901
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wayne Materi
Date created: 2008-10-23 11:00:00
Date modified: 2015-05-08 01:08:46

TA1 (THRU gate)



Types
DnaRegion

Roles
Terminator

terminator

Sequences BBa_K102901_sequence (Version 1)

Description

TA1 is based on the canonical his terminator (Larson et al, 2008) with all U's at the 3' tail.

Notes

Gate is flanked by BamHI and NcoI to facilitate simple replacement in output test harness. Because gates are fairly long duplex DNA strands, a single strand can be ordered and the dsDNA created by PCR using Term+ (5'cgatcagaattcgcggcc) and Term- (5'GGACATCTGCAGCGGCCG) primers.

Source

(Larson et al, 2008)

Sequence Annotation Location Component / Role(s)
his term
19,58
feature/misc sequence_feature
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K102901/1