| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1033115_sequence (Version 1)
|
Description
This is a mutant of E. coli's native thioredoxin. It has been modified to have the ability to chelate metal ions, notably nickel. This protein is useful as a fusion protein which can help to increase the solubility of difficult-to-express proteins and maximize yield of those proteins. Please note that this protein does not have a stop codon, to aid in its use as a fusion protein.
Notes
This construct was synthesized as a gblock by Integrated DNA Technologies (IDT) and then cloned into pSB1C3
Source
E.coli
