BBa_K1033120

BBa_K1033120 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1033120

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Magnus Bergman, Pontus Danielsson, Alexander Wirtanen, Nils Anlind, Christoffer Frisk, Thorsteinn Olaufsson, Malin Borg, Niclas Sandberg

Date created: 2013-09-01 11:00:00

Date modified: 2015-06-11 08:28:53

B0034-ExpTag-Linker-Miraculin-Linker-6xHis

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1033120_sequence (Version 1)

Description

Construct needed to produce the protein miraculin, but fitted with a, export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Notes

The miraculin sequence is codon optimized for E.coli. The codon usage for the export tag USP45's is best suited for Lactobacillus strains

Source

Richadella Dulcifica, but codon optimized for E.coli only the miraculin is codon optimized. The codon usage for the export tag USP45's is best suited for Lactobacillus strains

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
USP45	18,99	tag, feature/tag
B0034	1,12	ribosome_entry_site, feature/rbs
Linker	682,690	sequence_feature, feature/misc
Linker	100,108	feature/misc, sequence_feature
His-tag	691,714	tag, feature/tag
Miraculin	109,681	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1033120/1

Member of these Collections

• iGEM Parts Registry