| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1033259_sequence (Version 1)
|
Description
This is a toxin-antitoxin system originating from plasmid p256 from lactobacillus plantarum strain 256. (ref) It has been created without the natural promotor to allow for customisable strength of the system. The main use of the toxin-antitoxin system is to make the plasmid more stabile without having to rely on antibiotic resistance. The antitoxin has a shorter halflife then the antitoxin. If the cell were to lose the plasmid the toxin will remain longer in the cell and thereby kill it. It has been proven very efficient in previous studies. (ref)
Notes
The system has been amplified without RBS and its natural promotor. Since that promotor has not been characterized as far as we know, several promoters will probably have to be tried to find one that provides stability without adding to much cost of expressing the toxin-antitoxin. There is also a version available with the natural promoter and RBS.
Source
Lactobacillus plantarum 256
