| Types || |
| Roles |
| Sequences || BBa_K1039000_sequence (Version 1)|
DescriptionThe attP site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).
SourceGroth, A. C., Olivares, E. C., Thyagarajan, B. & Calos, M. P. (2000). A phage integrase directs efficient site-specific integration in human cells. Proc Natl Acad Sci U S A 97, 5995-6000.