BS2

BBa_K1039002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1039002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aaron Bender
Date created: 2013-07-07 11:00:00
Date modified: 2015-05-08 01:08:50

Bxb1 J23118 promoter switch



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K1039002_sequence (Version 1)

Description

The medium strength constitutive promoter J23111 complementary sequence is flanked by the Bxb1 attP and the complementary sequence of the Bxb1 attB site. In the default state the promoter directs transcription on the negative sense strand towards the attP site. In the presence of the Bxb1 integrase the attP and attB sites are recombined and the promoter is directing transcription to the sense strand towards the attB site.

Notes

The transcriptional terminator J61048 was included to ensure transcription only proceeds in the expected direction. Spacer regions were added adopted from ... NheI site ...

Source

Ghosh, P., Kim, A. I. & Hatfull, G. F. (2003). The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB. Mol Cell 12, 1101-1111.

igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1039002/1