| Types | DnaRegion
|
| Roles | DNA
sequence_feature
|
| Sequences | BBa_K1039002_sequence (Version 1)
|
Description
The medium strength constitutive promoter J23111 complementary sequence is flanked by the Bxb1 attP and the complementary sequence of the Bxb1 attB site. In the default state the promoter directs transcription on the negative sense strand towards the attP site. In the presence of the Bxb1 integrase the attP and attB sites are recombined and the promoter is directing transcription to the sense strand towards the attB site.
Notes
The transcriptional terminator J61048 was included to ensure transcription only proceeds in the expected direction. Spacer regions were added adopted from ... NheI site ...
Source
Ghosh, P., Kim, A. I. & Hatfull, G. F. (2003). The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB. Mol Cell 12, 1101-1111.
