Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: NRP UEA
Date created: 2013-08-07 11:00:00
Date modified: 2015-05-08 01:08:51
RFP Coding Device
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1041000_sequence (Version 1) |
Description
Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid.Notes
Nde1 site was added at the start of the RFP coding region.Source
BBa_J04450| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 end of LacI coding region (inactive) CAP binding site -35 -10 LacI binding site start conserved BBa_B0034 mrfp1 Help:Barcodes BBa_E1010 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 Nde1 Restriction Site | 1,200 1,88 89,126 137,142 161,166 166,200 173,173 213,216 209,220 227,901 908,932 227,932 941,1020 952,995 1029,1069 1036,1055 1056,1069 1062,1062 941,1069 224,229 | feature/BioBrick engineered_region CDS feature/cds non_covalent_binding_site feature/binding promoter feature/promoter promoter feature/promoter feature/binding non_covalent_binding_site start_codon feature/start feature/conserved conserved_region feature/BioBrick engineered_region CDS feature/cds engineered_tag feature/barcode engineered_region feature/BioBrick engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop feature/polya polyA_site stop_codon feature/stop engineered_region feature/BioBrick sequence_feature feature/misc |