Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: NRP UEA
Date created: 2013-08-07 11:00:00
Date modified: 2015-05-08 01:08:51
Neomycin Resistance Coding Device + AntG promoter
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1041001_sequence (Version 1) |
Description
Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid.Notes
Nde1 site was added at the start of the RFP coding region.Source
BBa_J04450| Sequence Annotation | Location | Component / Role(s) |
| S. S4 AntG Promoter neomycin resistance gene (optimised) rbs Nde1 site | 24,62 129,922 113,121 126,131 | promoter feature/promoter CDS feature/cds feature/rbs ribosome_entry_site feature/misc sequence_feature |