BBa_K1042015

BBa_K1042015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1042015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Parth Patel

Date created: 2013-09-25 11:00:00

Date modified: 2015-05-08 01:08:51

Cadmium Responsive GFP+Ogr Activator

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1042015_sequence (Version 1)

Description

After creating two intermediate parts, the upstream one being the Cadmium Promoter with Ogr Activator (BBa_K1042012) and the downstream one being the PO Promoter with GFP (BBa_K1042013), we digested the intermediates and ligated them to the linearized pSB1C3 plasmid provided. The resulting colonies were tested for responsive GFP production from the addition of cadmium.

Notes

test

Source

test

Components

Access	Instance	Definition
public	BBa_K1042012	BBa_K1042012
public	BBa_K1042013	BBa_K1042013

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1042012	1,450	BBa_K1042012
BBa_K1042013	459,1413	BBa_K1042013

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1042015/1

Member of these Collections

• iGEM Parts Registry