Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team G??ttingen 2013
Date created: 2013-06-23 11:00:00
Date modified: 2015-05-08 01:08:52
DarR ORF with inversed Pre- and Suffix
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1045001_sequence (Version 1) |
Description
This is the ORF of DarR. DarR is a regulatory protein from Mycobacterium smegmatis, which can bind its specific binding sequence (the DarR_operator, see part BBa_K1045000). The binding effinciency is dependent on the availability of cyclic di-AMP, which enhances the probability of binding to DNA. Ideally, this protein will shut down transcription of information downstream of its operator.This way, transcription can be controlled via the level of c-di-AMP (e.g. for a reporter-system in order to monitor it).
Notes
In order to express DarR in Escherichia coli, the GTG-start codon was mutated to an ATG. Also, the first few codons were changed according to codon usage in E. coli via mutagenesis PCR (with the forward primer).Source
This part was amplified from the genomic DNA of Mycobacterium smegmatis MC2 155.| Sequence Annotation | Location | Component / Role(s) |
| DarR reverse | 1,639 | CDS feature/protein |