DacA

BBa_K1045003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1045003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM Team G??ttingen 2013

Date created: 2013-08-21 11:00:00

Date modified: 2015-05-08 01:08:52

Diadenylate cyclase domain of Listeria monocytogenes cdaA (DacA)

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K1045003_sequence (Version 1)

Description

Cyclic di-AMP is involved in cell signaling of gram positive bacteria including L. monocytogenes, B. subtilis, S. aureus, and S. pneumoniae.
The diadenylate cyclase domain of Listeria monocytogenes cdaA protein generates cyclic di-AMP for cell signaling. The part BBa_K1045003 described here covers the amino acids 100 to 273 of the full length coding sequence of cdaA.

Notes

The genomic sequence of the part BBa_K1045003 contained a SpeI restriction site on position 456 of the DNA sequence. The restriction site was deleted without altering the amino acid sequence of the protein.

Source

The part was amplified using pcr from L. monocytogenes genomic DNA.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start	1,3	feature/start, start_codon
stop	526,528	stop_codon, feature/stop
Diadenylate cyclase domain	1,528	feature/protein, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1045003/1

Member of these Collections

• iGEM Parts Registry