BBa_K105001

BBa_K105001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K105001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Manuel Gersbacher

Date created: 2008-10-11 11:00:00

Date modified: 2015-05-08 01:08:52

VP16 - eucaryotic activation domain

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K105001_sequence (Version 1)

Description

Originally VP16 is a protein from the herpes simplex virus. It activates the transcription of the late genes of this virus by the RNA-polymerase II of the eucaryotic host.

In artificial systems VP16 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective expression of genes with a promoter containing this bait sequence can be achieved.

Notes

To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick.
For more information about this issus, see Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

We have constructed our BioBrick from the plasmid xxx, which was available in our hosting lab.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K105001/1

Member of these Collections

• //cds/transcriptionalregulator/activator
• //chassis/eukaryote
• iGEM Parts Registry