BBa_K105002

BBa_K105002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K105002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sandra Dittmann

Date created: 2008-10-11 11:00:00

Date modified: 2015-05-08 01:08:52

TUP1 - repressor domain for transcription factors in yeast

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K105002_sequence (Version 1)

Description

This BioBrick is the N-terminal repression domain of the yeast general repressor TUP1.
In artificial systems TUP1 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective repressionn of genes with a promoter containing this bait sequence can be achieved.

Notes

To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick.

For more information about this issus, see Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." - DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

Yeast genomic DNA.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K105002/1

Member of these Collections

• //cds/transcriptionalregulator/repressor
• //chassis/eukaryote/yeast
• iGEM Parts Registry