BBa_K105003

BBa_K105003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K105003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Katja Karstens

Date created: 2008-10-11 11:00:00

Date modified: 2015-05-08 01:08:52

tetR - DNA binding domain

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K105003_sequence (Version 1)

Description

The TetR protein is a repressor that binds to a specific operator site ([[BBa_R0040]] & [[BBa_K105020]]). This site specific binding can be inhibited by the small molecule tetracyclin and analogs of tetracyclin.

Notes

To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.
The frist codon has been changed from TCC to AGT (both Ser) to prevent a methylation site.


For more information about this issus, see:

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering."
DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

We changed the format of the TetR coding part of BBa_C0040 into Phillips/Silver standard.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K105003/1

Member of these Collections

• //proteindomain/dnabinding
• iGEM Parts Registry