cln2

BBa_K105014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K105014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Robin Sorg

Date created: 2008-10-18 11:00:00

Date modified: 2015-05-08 01:08:52

cln2 PEST destabilization domain for rapid protein turnover

Details

• Types: DnaRegion
• Roles: tag; Tag
• Sequences: BBa_K105014_sequence (Version 1)

Description

This BioBrick is the xxx domain of the yeast cln2 protein. It has been shown that this region of part of the protein is suffient to get xxx specific degradation via the APC complex.

Notes

To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.


For more information about this issus, see:

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering."
DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

Yeast genomic DNA

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K105014/1

Member of these Collections

• //proteindomain/degradation
• iGEM Parts Registry