Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Wild, Katja Karstens
Date created: 2008-10-21 11:00:00
Date modified: 2015-05-08 01:08:52
Ura3 - Orotidine-5-phosphate decarboxylase
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K105017_sequence (Version 1) |
Description
The Orotidine-5'-phosphate (OMP) decarboxylase catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines in S. cerevisiae. It converts OMP into uridine monophosphate (UMP) and capable to transform 5-FOA into the toxic 5-fluorouracil. Under the name of Ura3 it is one of the commonly used auxotrophic markers in Yeast. There exists a number of S. cerevisiae strains which are deleated for this enzyme.Our BioBrick contains the protein coding sequence as well as the natural promoter and terminator of the gene. To avoid a transcription of genes downstream of this part, it might be usefull put a strong termintor (i.e. Adh1_term: [[Part:BBa_J63002| BBa_J63002]]) in between the parts.
Notes
Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.So there is no base in between the BioBrick and the restriction sites.
The deletation of an internal Pst1 site has been possible with a silent mutation.
Source
Amplification from the plasmid pRS416 (Sikorski & Hieter)| Sequence Annotation | Location | Component / Role(s) |
| Orotidine-5'-phosphate decarboxylase please mutate T -> C | 232,1036 211,211 | CDS feature/cds point_mutation feature/s_mutation |