BBa_K105030

BBa_K105030 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K105030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michael Wild

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:08:52

cyc28 minimal promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K105030_sequence (Version 1)

Description

This is a derivate of [[Part:BBa_K105027| BBa_K105027]]. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 28 % of the natural yeast CYC1 promoter.

Notes

This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!



Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.

Source

Amplification and Mutation from the plasmid pAH3

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
TATA-box	17,23	sequence_feature, feature/misc
A -> T	22,22	feature/s_mutation, point_mutation

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K105030/1

Member of these Collections

• //chassis/eukaryote/yeast
• //direction/forward
• //promoter
• //regulation/constitutive
• //rnap/eukaryote/yeast
• iGEM Parts Registry