BBa_K105101

BBa_K105101 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K105101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Katja Karstens

Date created: 2008-08-11 11:00:00

Date modified: 2015-05-08 01:08:53

Gal4 - DNA binding domain

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K105101_sequence (Version 1)

Description

This is the DNA binding domain of the Gal4 repressor. Its binding to the operator BBa_K105xxx is no longer dependent on galatose.
Our Gal4 - DNA binding domain does not contain neither start nor stop codon.

Notes

To enable the fusion with other domains in frame the vector of this BioBricks has no base pair in between the restriction side and the BioBrick (Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

plasmid from the local lab containing a Gal4-VP16 chimera

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K105101/1

Member of these Collections

• iGEM Parts Registry