BBa_K1051304

BBa_K1051304 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1051304

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kequan Lin

Date created: 2013-06-29 11:00:00

Date modified: 2015-05-08 01:08:54

cln3 promoter (M phase to G1 phase)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1051304_sequence (Version 1)

Description

It is a promoter which promotes transciption in M of the yeast cell cycle .If you want to express your proteins in M of the yeast cell cycle ,you can add the sequence which is actived by cln3 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .

Notes

We get the sequence which is actived by cln3 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows :
upstream:
downstream:

Source

GAAAAATTATCAGGCAAGAAAAAGAAATTACCAAGCCTGCTCTCACTGTAATGATCAAGT
TACATAAATTTACTATCGGATTAGTGTGTCCCTCTGCCACATTTCCATATTTGGCCTTGG
GTTTTTGCCCTCATCTTTTTTTTTTCTTCCTCCTACCTATTTATAATTTGTATATCTGTA
CTTTCCTCGAGCTTTTAATCTTCTTCTTAAACATTTTCTTGTGTAGTATACTTTCACACA
ATTTCTTTCTTGATTTTTTCTTCTACTACTGAGTCTGCCAGTCAAATGGATTTCTGAGGA
AAGAGGACTATACCCATTAGGAAACGAATTGCCCGAGTAGTCTCCTCTGCCGACTTAAAC
CAACCTTTTTCTATTTCTCTTTTCTTTTCTCCCTCTTTTTTCTCTGTACTAGCATCCAAA
AGCAAGCATCCATCCGAGTCCCAGTCGCAATCTCACATCTCCAATTTAACGTATCCATTG
CATTTCCTCATTCGGTTTAACTCCTCTGCATTTCTTTTCTGACCCATAGCATTTCTTACA
TTCCATTGCATCTCCCTTTTACTCTCGTTCAAGACACTGATTTGATACGCTTTCTGTACG

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1051304/1

Member of these Collections

• iGEM Parts Registry