BBa_K1053014

BBa_K1053014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1053014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ippei Sakamoto
Date created: 2013-09-26 11:00:00
Date modified: 2015-05-08 01:08:56

TR(12)-HHR-taR12y



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K1053014_sequence (Version 1)

Description

This hammerhead ribozyme(HHR) has trans-acting RNA(taRNA) responsive region, and this HHR responses to taR12. In absence of taR42, the HHR can self-cleave, but in presence of taR12, their capability of self-cleavage is inhibited due to hybridize with taR12 and change their structures. This HHR has taR12y in the downstream. taR12 and taR12y are orthogonal riboregulators reported by Callura et al.

Notes

The region recognizing TR-HHR in taRNA is sequestrated in HHR's extended stem I.

Source

This is composed of BBa_K1053000(taRNA responsive Hammerhead Ribozyme(TR-HHR) with RBS) & BBa_K1053010(PBAD-taR12y-DT)

Sequence Annotation Location Component / Role(s)
taR12y
TR(12)-HHR
94,164
14,100
feature/misc sequence_feature
feature/misc sequence_feature
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1053014/1