BBa_K1059002

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K1059002_sequence (Version 1)

This device consists of a GFP with LVA tag under the control of B0035. And at the end of the part, there???s a terminator named B0015.

The GFP-LVA is from part K145280, but it is reported that the RBS is so weak that it cannot work well, so we have to change the RBS to B0035 by PCR.

The GFP-LVA sequence is from part K145280, we change the RBS B0032 to B0035 and standardize it by PCR .

Sequence Annotation	Location	Component / Role(s)
rbs GFP-LVA start stop codon B0015	4,23 24,779 24,26 774,779 791,919	feature/rbs ribosome_entry_site CDS feature/cds start_codon feature/start stop_codon feature/stop stem_loop feature/stem_loop