Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Qiu Wang
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:08:57
Its transcript can prevent the mRNA itself from being degraded by RNaseE.
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1059003_sequence (Version 1) |
Description
This coding cosists of a RBS ,a start codon and several stop codons in different reading frames. It can be used in the 5'-UTR of a mRNA, preventing the mRNA degradation mediated by RNaseE.The extra RBS can attract ribosome to bind on it.And it has the ability to prevent the degradosome from being actived by 5' ribonucleotide because of it???s steric hindrance.The start codon makes binding more efficient and the stop codon just prevents the DNA???s translation.Notes
To make sure the 5' ribonucleotide protected by a ribosome,we must arrange the RBS close to the 5' extreme ribonucleotide.And also ,the "extra RBS " and the "real RBS " must be enough interval, preventing the ribosome interfe with each other.Source
This segment is design by oueselves.| Sequence Annotation | Location | Component / Role(s) |
| promoter B0035 start codon stop codon stop codon stop codon | 1,35 43,49 56,58 59,64 66,68 70,72 | promoter feature/promoter feature/rbs ribosome_entry_site feature/start start_codon stop_codon feature/stop stop_codon feature/stop feature/stop stop_codon |