BBa_K1059004

BBa_K1059004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1059004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Qiu Wang
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:08:57

Its transcript can prevent the mRNA itself from being degraded by exonuclease.



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1059004_sequence (Version 1)

Description

This coding cosists of a RBS ,a start codon and several stop codons in different reading frames. It can be used in the 3'-UTR of a mRNA, preventing the mRNA degradation mediated by exonuclease. The extra RBS can attract ribosome to bind on it,which can prevent the mRNA from being degraded by exonuclease . The start codon makes binding more efficient and the stop codon just prevents the DNA???s translation.

Notes

The "extra RBS " and the "real RBS " must be enough interval, preventing the ribosome interfe with each other.

Source

This segment is design by oueselves.

Sequence Annotation Location Component / Role(s)
B0035
start codon
stop codon
28,41
48,50
51,56
feature/rbs ribosome_entry_site
start_codon feature/start
stop_codon feature/stop
igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1059004/1