BBa_K1059005

BBa_K1059005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1059005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xue Sun

Date created: 2013-08-28 11:00:00

Date modified: 2015-05-08 01:08:57

DNA segment whose transcript can prevent mRNA degradation by RNaseE.

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1059005_sequence (Version 1)

Description

This segment consists of two RBS,a start codon and several stop codon in different reading frames. We think it will be higher efficient in protecting mRNA from degradation.
It is used in the 5'-UTR of mRNA, preventing mRNA degradation mediated by RNaseE.The extra RBS can binding ribosome, preventing RNaseE or the degradosome actived by 5' ribonucleotide.The start codon is for more efficient binding and the stop codon prevents translation.

Notes

The "extra RBS " and the "real RBS "there must be enough interval, preventing the ribosome interfereing with each other.

Source

This segment is designed by ourselves.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
J23101	1,29	promoter, feature/promoter
B0035	43,49	ribosome_entry_site, feature/rbs
B0035	120,126	ribosome_entry_site, feature/rbs
start	163,165	start_codon, feature/start

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1059005/1

Member of these Collections

• iGEM Parts Registry