| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1059014_sequence (Version 1)
|
Description
mamK is a protein coding squence from Magnetospirillum magneticum AMB-1 bacteria strain, mamAB gene cluster. It will guide the construction of MMB with mamI, mamB, mamL, mamQ, which we use to construct the intracellular compartment.
Notes
Because there is a PstI cut site, so we process point mutation by over-lap PCR.synthetic biology proposes standard prefix and sufix, so we add prefix and sufix by PCR before we ligease it onto plasmid PSB1C3.
Source
It comes from Magnetospirillum magneticum AMB-1 bacteria strain.We firstly design primers to get this gene squence by PCR from genome, and then use another primer to add RBS J23106, prefix and sufix onto it.
