| Types | DnaRegion
|
| Roles | Composite
engineered_region
|
| Sequences | BBa_K1059027_sequence (Version 1)
|
Description
This coding cosists of a RBS ,an examing protein GFP-LVA,a start codon and several stop codons in different reading frames. It can be used in the 3'-UTR of a mRNA, preventing the mRNA degradation mediated by exonuclease. The extra RBS can attract ribosome to bind on it,which can prevent the mRNA from being degraded by exonuclease . The start codon makes binding more efficient and the stop codon just prevents the DNA???s translation. The circuit is on the backbone of pSB1AK3.We can test the efficiency of the BBa_K1059004 through this circuit.
Notes
The "extra RBS " and the "real RBS " must be enough interval, preventing the ribosome interfe with each other.And we can test the efficiency of the BBa_K1059004 through this circuit.
Source
The GFP-LVA is from K145015.
