Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yu Wang
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:08:57
GFP-LVA under RNA guardian control
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1059066_sequence (Version 1) |
Description
This device consists of a GFP with LVA tag under the control of B0035. At the 3???-UTR and 5???-UTR of the part , we add part K1059004 and K1509001 to stabilize its mRNA to improve the expression of the gene. The end of the gene is terminator B0015.Source
The GFP-LVA sequence is from part 145280, but we change the RBS. And we add K1509004 between B0015 and GFP-LVA, add k1509001 before GFP-LVA.| Sequence Annotation | Location | Component / Role(s) |
| J23101 B0035 GFP-LVA start codon stop codon K1059004 B0015 | 1,35 46,65 66,821 66,68 816,821 833,919 928,1056 | promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/cds start_codon feature/start feature/stop stop_codon feature/dna sequence_feature feature/stem_loop stem_loop |