Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Horwitz
Date created: 2008-10-28 12:00:00
Date modified: 2015-05-08 01:08:59
AarI AD acceptor vector (pRS305, Gal1P, Adh1t)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K106672_sequence (Version 1) |
Description
This is an acceptor vector for AarI cloning, with A and D ends. Cloning into this plasmid will put your parts under the control of a Gal1 promoter (Gal1P) and Adh1 terminator (Adh1t). For more information regarding AarI acceptor vectors, and AarI cloning in general, please visit the UCSF 2008 wiki.Notes
The pRS305 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites.Please note, the actual orientation of this vector is reversed.
Source
A modified pRS305 plasmid--Integrating yeast expression vector (LEU2 marker).| Sequence Annotation | Location | Component / Role(s) |
| LEU2 marker ampicillin resistance marker Gal1 Promoter Adh1 terminator D overhang T7 promoter lacZalpha reporter f1 origin ampicillin resistance promoter pBR322 origin lac promoter T3 promoter Gal1 promoter Stop codons in all 3 frames Start codon A overhang | 1570,2676 3539,4399 5618,6634 30,215 1,4 224,242 246,405 427,733 3469,3497 4554,5173 5482,5511 5581,5600 6172,6621 11,21 6626,6628 6635,6638 | feature/cds CDS feature/cds CDS feature/promoter promoter feature/stem_loop stem_loop sequence_feature feature/dna feature/promoter promoter feature/misc sequence_feature sequence_feature feature/dna promoter feature/promoter feature/dna sequence_feature promoter feature/promoter promoter feature/promoter feature/promoter promoter feature/stop stop_codon feature/start start_codon sequence_feature feature/dna |