| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K106692_sequence (Version 1)
|
Description
Sir3 is the silencing protein that recognizes de-acetylated histone tails, and in combination with Sir4, recruits more Sir2 to propagate spreading of silencing.
Notes
Sir3 does not tolerate amino-terminal fusions, so we have redesigned the AarI A site. The new site is termed A!, introduces no additional amino acids at the N-terminus, and only works for cloning into an A! acceptor vector. For more information, please see the UCSF 2008 iGEM wiki.
Source
Sir3 was PCR amplified from yeast genomic DNA.
