Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Horwitz
Date created: 2008-10-29 12:00:00
Date modified: 2015-05-08 01:09:00
AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t)
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K106698_sequence (Version 1) |
Description
Acceptor for AarI AD parts, with 8X LexA operators directly upstream of the medium strong Cyc1 promoter.Notes
LexA Ops were cloned non-directionally into the PspOMI site. Inner site is broken, thus the cassette can be subcloned from this plasmid by PspOMI/SacI digestion (for example, to change markers).Source
Modification of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t).| Sequence Annotation | Location | Component / Role(s) |
| CEN ARS ampicillin resistance marker 8X LexA Operators Fig1 Promoter Adh1 terminator LEU2 marker T7 promoter lacZalpha reporter f1 origin ampicillin marker promoter pBR322 origin lac promoter T3 promoter D overhang Stop codons in all 3 frames A overhang | 3403,3921 4053,4913 6132,6299 6300,7309 22,215 1570,2676 224,242 246,405 427,733 2983,4011 5068,5687 5996,6025 6095,6114 1,4 11,21 7309,7312 | feature/misc sequence_feature feature/cds CDS feature/operator operator promoter feature/promoter feature/stem_loop stem_loop feature/cds CDS feature/promoter promoter feature/misc sequence_feature sequence_feature feature/dna feature/promoter promoter feature/dna sequence_feature promoter feature/promoter feature/promoter promoter feature/dna sequence_feature stop_codon feature/stop sequence_feature feature/dna |